Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Salvia miltiorrhiza injection ameliorates myocardial ischemia-reperfusion injury via downregulation of PECAM-1

Yang Ronghai¹, Yao Weiping², Liu YingFeng¹ , Wang Xuejun³, Liang Jianguang⁴, Liu Jiachao⁴

¹Department of Cardiovascular Medicine, Zhujiang Hospital of Southern Medical University, Guangzhou 510280; ²Department of Respiration Medicine; ³Department of Neurology; ⁴Department of Cardiovascular Medicine, People's Hospital of Sanshui District, Foshan City, Foshan 528100, Guangdong, China.

For correspondence:- Liu YingFeng Email: liuyingfeng034@aliyun.com

Accepted: 15 June 2019 Published: 28 July 2019

Citation: Ronghai Y, Weiping Y, YingFeng L, Xuejun W, Jianguang L, Jiachao L. Salvia miltiorrhiza injection ameliorates myocardial ischemia-reperfusion injury via downregulation of PECAM-1. Trop J Pharm Res 2019; 18(7):1467-1473 doi: 10.4314/tjpr.v18i7.15

© 2019 The authors.
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Abstract

Purpose: To investigate the effect of Salvia miltiorrhiza injection on myocardial ischemia-reperfusion injury and PECAM-1 related pathways.

Methods: Male Wistar rats were used for establishment of myocardial ischemia-reperfusion model. The rats were randomly assigned to four groups: experimental group, low dose group (Salvia miltiorrhiza injection, 10 mL/kg/day), moderate dose group (Salvia miltiorrhiza injection, 20 mL/kg/day) and high dose group (Salvia miltiorrhiza injection, 40 mL/kg/day). Myocardial ischemia-reperfusion model was established in the four groups. Evans-TTC staining was used to assess relative area of ischemia-reperfusion injury. Blood samples were collected for assay of PECAM-1 expression using enzyme-linked immunosorbent assay (ELISA). Fresh blood platelets were collected in all groups, and divided into two groups - control group (normal culture) and experimental group (Salvia miltiorrhiza injection). The expression of PECAM-1 in blood platelets was assayed using Western blot.

Result: Compared with the experimental group, Salvia miltiorrhiza injection ameliorated myocardial ischemia-reperfusion injury, and decreased the infarction area seen in Evans/TTC staining. PECAM-1 expression in blood was decreased by Salvia miltiorrhiza injection. Blood platelets dysfunction was induced after myocardial ischemia-reperfusion, and the level of PECAM-1 increased. However, Salvia miltiorrhiza injection treatment downregulated the expression of PECAM-1 after myocardial ischemia-reperfusion.

Conclusion: Salvia miltiorrhiza injection maintains normal function of blood platelets and ameliorates myocardial ischemia-reperfusion injury by decreasing expression of PECAM-1.

Keywords: Salvia miltiorrhiza, PECAM-1, Blood platelets, Myocardium, Ischemia-reperfusion